ELISA Unit

We carry out enzyme-linked immunosorbent assays (ELISA) using Architect i2000SR analyzer:

• Extended panel of viral hepatitis markers;
• Markers of viral diseases (cytomegalovirus, rubella, toxoplasmosis, herpes simplex, Epstein-Barr virus);
• Tumor markers;
• Thyroid hormones;
• Markers of autoimmune and rheumatic diseases (8 parameters);
• Markers of autoimmune hepatitises (6 parameters);
• Markers of an acute coronary syndrome;
• Tests for diabetes mellitus;
• Therapeutic drug monitoring.

Equipment:

• Architect i2000SR analyzer;
• MiniVIDAS/Blue analyzer;
• Multiscan MS analyzer.

List of parameters:

1. Marker of autoimmune diseases:

• Anti-CCP, cyclic citrullinated peptide antibody;
• Anti-ds DNA antibodies;
• Ig G antinuclear antibodies;
• Anti-Sm antibodies;
• Sm/RNP;
• SSA RO;
• SSB LA;
• ANCA.

2. Marker of autoimmune hepatitis:

• Ig G ANA;
• Anti-LKM₁;
• AMA M₂;
• Anti-Sp-100;
• SLA Ig G.

3. Markers of herpes virus infections:

• HSV I + II Ig G;
• HSV I + II Ig M;
• VZV Ig G;
• CMV Ig G;
• CMV Ig M;
• VCA/EA Ig G;
• EBNA Ig G;
• VCA Ig M.

4. Antiphospholipid antibodies:

• Cardiolipin Ig M;
• Cardiolipin Ig G;
• β2-Glycoprotein I Ig M;
• β2-Glycoprotein I Ig G.

5. TORCH panel:

• Toxo Ig G;
• Toxo Ig M;
• Rubella Ig G;
• Rubella Ig M;
• CMV Ig G;
• CMV Ig M;
• Ig G Chlamydia trachomatis;
• Ig M Chlamydia trachomatis;
• Ig A Chlamydia trachomatis.

6. Marker of viral hepatitises:

• Hepatitis B markers

• B HbsAg;
• HBsAg confirm;
• anti-HBs;
• HbcAg;
• Anti-HBe;
• Ig M Anti-HBc;
• Ig M + Ig G anti-HBeIgM

• Hepatitis C markers

• HCVcAg;
• IgM + IgG anti-HCV

• Hepatitis A markers

• HAVAb Ig M;
• HAVAb Ig G.

7. Thyroid hormones:

• TSH thyroid-stimulating hormone;
• FT4 free thyroxin;
• FT3 free triiodothyronine;
• TT4 total thyroxine;
• TT3 total triiodothyronine;
• Anti-TPO thyroperoxidase antibodies.

8. Tumor markers:

• CEA;
• AFT;
• CA 19-9;
• CA 15-3;
• CA 125;
• HE 4;
• Total PSA;
• Free PSA;
• Total β-hCG;
• SCC;
• ProGP;
• Cyfra 21.1.

9. Cardiac markers:

• Troponin I;
• Homocysteine;
• Myeloperoxidase;
• B-Type Natriuretic Peptide.

10. Metabolites:

• Vitamin B₁₂;
• Folic acid;
• Insulin;
• C-peptide;
• NGAL;
• Cortisol;
• i-PTH Intact parathyroid hormone.

11. Medical drug monitoring:

• Tacrolimus;
• Ciclosporin;
• Vancomycin.

12. Markers of sepsis:

• Procalcitonin PCT